TEST FOR BILIRUBIN
What is bilirubin? Bilirubin is a yellowish substance in your blood. It forms after red blood cells break down, and it travels through your liver, gallbladder, and digestive tract before being excreted. What are the causative agents of change in bilirubin level (high/low)? Following are the causative agents of bilirubin; Gallstones Gilbert’s Syndrome Liver dysfunction Hepatitis Bile duct inflammation Intrahepatic cholestasis of pregnancy Hemolytic anemia GILBERT'S SYNDROME IS A GENETIC DISORDER THAT INVOLVE THE BELOW GIVEN SYMPTOMS ASSOCIATED WITH IT Biochemistry test for bilirubin detection; For detection of level of bilirubin in body 3 test are commonly used; Total bilirubin Direct bilirubin Indirect bilirubin Direct bilirubin: In direct bilirubin simply reagents are used & then analyzed by using biochemistry analyzer. The procedure is; Add 200ul serum in test tubes labelled as TEST & BLANK. Add 200ul reagent-1 from bilirubin regent kit, in both test tube Add 50ul...